Shaheed Zulfikar Ali Bhutto Institute of Science and Technology, Pakistan
Title: Comparative inhibitory :Effect of Camiella sinensis (green tea) aqueous non-fermented, fermented and Semi fermented extracts against H. pylori tested on Pregnant women in Karachi
Kiran Fatima Lab Technician at Department of Biosciences Sheheed Zulfikar Ali Bhutto Insititute Of Sciences & Technology (SZABIST) karachi campus Pakistan,has done Master in Physiology from University Of Karachi.She had been a Visiting Faculty in SZABIST she is also working in different reearch project like,isolation,chahracterization and purification of various enzymes from microbial source.
Background and objectives: Helicobacter pylori, was found to be an important factor in the pathogenesis of peptic ulcer disease, gastritis, and gastric malignancy. The growing problem of antibiotic resistance by the organism in Pakistan thus demands the search for novel compounds, especially from natural sources as in this study; we screened the aqueous extracts of Camiella sinensis and also compared the anti bacterial efficacy of its non-fermented, semi -fermented and fermented products. Methods: All these biopsies were processed for detection of H.pylori by two rapid Helicourease – indigenously developed rapid urease detection kits, culture and polymerase chain reaction (PCR).The 5% aqueous extract of fermented, semi fermented and non-fermented Green tea was prepared and their antibacterial potential was explored against 35 clinical isolates of H. pylori agar well diffusion technique. The Minimum Inhibitory Concentration (MIC) of the most susceptible tea products were also carried out by Micro-dilution method for the sake of comparison. Results: Most of the screened H .pylori isolates were resistant to more than one of the antibiotics like metronidazole and clarithromycin. Significant activity was obtained in non-femented green tea with an average zone of inhibition along with MIC was around 32 mm , semi fermented product showed 28 mm.Conclusion:Results indicated that all forms of G. tea extracts possessed some variable level of anti H. pylori activity. But non fermented that is freshly plucked g.tea leaves with out any industrial treatment have profound effect with least MIC and thus considered as a suitable candidate for the eradication of H.pylori drug resistant species.